Dynamic BMP gene expression regulation in chick RPE during recovery from short term optical defocus and form-deprivation.

ABSTRACT:

Purpose

This study investigated the differential gene expression of BMPs in chick retinal pigment epithelium (RPE) during recovery from short term exposure to optical defocus and form-deprivation (FD) treatments.

Methods

14-day old White-Leghorn chicks wore either monocular +10 or -10 D lenses, or diffusers for 2 or 48 h, after which eyes were allowed unobstructed vision for up to 96 h. Over this recovery period, refractive errors and choroidal thickness (ChT) were tracked using retinoscopy and high-frequency A-scan ultrasonography. Real-time PCR was used to examine the expression of BMP2, 4, and 7 genes in RPE samples collected 0, 15 min, 2, 24, 48, and 96 h after the termination of treatments. Expression levels in treated eyes and their contralateral control eyes were comp

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11469538 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature