RBMS1 interference inhibits malignant progression of glioblastoma cells and promotes ferroptosis.

ABSTRACT:

Background

Glioblastoma (GBM) is a brain tumor characterized by the highest malignancy and the poorest prognoses. RNA binding motif single strand interacting protein 1 (RBMS1) has been implicated to be involved in various cancer progression. This study was conceived to explore the role and the mechanism of RBMS1 in GBM.

Materials

RT-qPCR and western blot were used to evaluate RBMS1 expression and examine the transfection efficiency of sh-RBMS1. Cell proliferation was detected using CCK-8 assay and colony formation assay while cell apoptosis was detected with flow cytometry. Cell migration and invasion were detected with wound healing and transwell assay. The activities of MMP2 and MMP9 were detected using gelatin zymography. Western blot was used to measure proliferation-, a

SUBMITTER: Liang X 

PROVIDER: S-EPMC11469991 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature