Identification of Haemoproteus infection in an imported grey crowned crane (Balearica regulorum) in China.

ABSTRACT: Blood parasites from the order Haemosporida infect many vertebrates and cause malaria-like diseases. In this study, a haemosporidian infection was detected in a sick grey crowned crane imported into China using a combination of morphological and molecular approaches. Blood samples were collected from the jugular vein and processed for morphological identification of infective parasites using stained blood smears and microscopy. No merogony occurs in the blood cells, and sporadic pigment granules were observed. Nested-PCR assays were employed for a molecular examination, which indicated that the cytb gene of this parasite had 94.1-94.9% identity to Haemoproteus antigonis. Subsequently, its mitochondrial genome structure was determined by high-throughput sequencing using the DNBSEQ-T7 platfo

SUBMITTER: Shen X 

PROVIDER: S-EPMC11469997 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature