Amplification-free detection of Ascochyta blight in chickpea using a simple molecular beacon assay.

ABSTRACT: Ascochyta blight is a major biotic stress that limits chickpea production globally. Fungicide application remains one of the effective control measures for the endemic spread. Due to the serious threat that synthetic fungicides pose to crop quality, early diagnosis of the pathogen is imperative. Whilst there have previously been several conventional lab-based diagnostic methods developed for early detection of Ascochyta rabiei, they require long assay times, specialised equipment and facilities, and trained personnel to process the samples. To overcome this challenge, a rapid amplification-free detection assay using a molecular beacon probe was developed. The method consists of a simple assembly assay that accurately detects pathogen within 30 min. The developed assay is species-specific a

SUBMITTER: Dyussembayev K 

PROVIDER: S-EPMC11470000 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature