A deployable film method to enable replicable sampling of low-abundance environmental microbiomes.

ABSTRACT: Urbanizing global populations spend over 90% of their time indoors where microbiome abundance and diversity are low. Chronic exposure to microbiomes with low abundance and diversity have demonstrated negative long-term impacts on human health. Sequencing-based analyses of environmental nucleic acids are critical to understanding the impact of the indoor microbiome on human health, however low DNA yields indoors, alongside sample collection and processing inconsistencies, currently challenge study replicability. This study presents a comparative assessment of a novel, passive, easily replicable sampling strategy using polydimethylsiloxane (PDMS) sheets alongside a representative swab-based collection protocol. Deployable, customizable PDMS films designed for whole-sample insertion into stan

SUBMITTER: Mankiewicz Ledins P 

PROVIDER: S-EPMC11470061 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature