Multicolor iLIFE (m-iLIFE) volume cytometry for high-throughput imaging of multiple organelles.

ABSTRACT: To be able to resolve multiple organelles at high throughput is an incredible achievement. This will have immediate implications in a range of fields ranging from fundamental cell biology to translational medicine. To realize such a high-throughput multicolor interrogation modality, we have developed a light-sheet based flow imaging system that is capable of visualizing multiple sub-cellular components with organelle-level resolution. This is possible due to the unique optical design that combines an illumination system comprising two collinear light sheets illuminating the flowing cells and a dedicated dual-color 4f-detection, enabling simultaneous recording of multiple organelles. The system PSF sections up to 4 parallel microfluidic channels through which cells are flowing, and multicol

SUBMITTER: Kumar P 

PROVIDER: S-EPMC11470118 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature