Structural insights into the initiation of free radical formation in the Class Ib ribonucleotide reductases in Mycobacteria.

ABSTRACT: Class I ribonucleotide reductases consisting of α and β subunits convert ribonucleoside diphosphates to deoxyribonucleoside diphosphates involving an intricate free radical mechanism. The generation of free radicals in the Class Ib ribonucleotide reductases is mediated by di-manganese ions in the β subunits and is externally assisted by flavodoxin-like NrdI subunit. This is unlike Class Ia ribonucleotide reductases, where the free radical generation is initiated at its di-iron centre in the β subunits with no external support from another subunit. Class 1b ribonucleotide reductase complex is an essential enzyme complex in the human pathogen Mycobacterium tuberculosis and its structural details are largely unknown. In this study we have determined the crystal structures of Mycobacter

SUBMITTER: Yadav LR 

PROVIDER: S-EPMC11470190 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature