A novel simple suture method for establishing an orthotopic pancreatic cancer mouse model: a comparative study with two conventional methods.

ABSTRACT:

Objective

This study aims to evaluate the efficacy of a novel simple suture method in establishing an optimal animal model for preclinical research in pancreatic cancer.

Methods

To establish a novel simple suture method, the tumor fragment was placed on the tail of the pancreas and securely wrapped into the pancreas, and compared with two conventional methods: the cell injection method and the tumor fragment embedding method. Subsequently, emission tomography/computed tomography scanning, gross anatomy observation, hematoxylin and eosin staining, and immunohistochemistry staining were performed to assess the effectiveness of these methods.

Results

The emission tomography/computed tomography scanning and anatomical examinations confirmed the successful construction of

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11470369 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature