Identification of crucial genes and possible molecular pathways associated with active vitamin D intervention in diabetic kidney disease.

ABSTRACT:

Background

A significant cause of advanced renal failure is diabetic nephropathy (DKD), with few treatment options available. Calcitriol shows potential in addressing fibrosis related to DKD, though its molecular mechanisms remain poorly understood. This research seeks to pinpoint the crucial genes and pathways influenced by calcitriol within the scope of DKD-related fibrosis.

Methods

Single-cell gene expression profiling of calcitriol treated DKD rat kidney tissue and screening of fibrosis-associated cell subsets. Mendelian randomization and enrichment analyses (CIBERSORT, GSVA, GSEA, Motif Enrichment) were used to explore gene-immune cell interactions and signaling pathways. Key findings were validated using independent datasets and protein expression data from the Human P

SUBMITTER: Zhang M 

PROVIDER: S-EPMC11470520 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature