Tau-mediated coupling between Pol III synthesis and DnaB helicase unwinding helps maintain genomic stability.

ABSTRACT: The τ-subunit of the clamp loader complex physically interacts with both the DnaB helicase and the polymerase III (Pol III) core α-subunit through domains IV and V, respectively. This interaction is proposed to help maintain rapid and efficient DNA synthesis rates with high genomic fidelity and plasticity, facilitating enzymatic coupling within the replisome. To test this hypothesis, CRISPR-Cas9 editing was used to create site-directed genomic mutations within the dnaX gene at the C terminus of τ predicted to interact with the α-subunit of Pol III. Perturbation of the α-τ binding interaction in vivo resulted in cellular and genomic stress markers that included reduced growth rates, fitness, and viabilities. Specifically, dnaX:mut strains showed increased cell filamentation, mutagenesis fre

SUBMITTER: Welikala MU 

PROVIDER: S-EPMC11470591 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature