Extracellular vesicles from human cardiac stromal cells up-regulate cardiomyocyte protective responses to hypoxia.

ABSTRACT:

Background

Cell therapy can protect cardiomyocytes from hypoxia, primarily via paracrine secretions, including extracellular vesicles (EVs). Since EVs fulfil specific biological functions based on their cellular origin, we hypothesised that EVs from human cardiac stromal cells (CMSCLCs) obtained from coronary artery bypass surgery may have cardioprotective properties.

Objectives

This study characterises CMSCLC EVs (C_EVs), miRNA cargo, cardioprotective efficacy and transcriptomic modulation of hypoxic human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). C_EVs are compared to bone marrow mesenchymal stromal cell EVs (B_EVs) which are a known therapeutic EV type.

Methods

Cells were characterised for surface markers, gene expression and differentiation

SUBMITTER: Czosseck A 

PROVIDER: S-EPMC11470622 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature