Whole genome analysis of Pantoea species identified from sepsis patients in selected Ethiopian referral hospitals: emerging pathogens.

ABSTRACT:

Background

The burden of sepsis worsens due to the continuation of emerging pathogens such as multidrug-resistant Pantoea species.

Methods

A multicenter study was conducted between October 2019 and September 2020 at four hospitals located in central, southern, and northern parts of Ethiopia. A total of 1416 sepsis patients were recruited and blood cultures were performed. At each study site, positive cultures were characterized by their colony characteristics, gram stain, and conventional biochemical tests. All Pantoea species were identified using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI TOF) and subjected to whole genome sequencing (WGS) using Illumina HiSeq 2500. The phylogeny structure of Pantoea isolates was calculated using IQ

SUBMITTER: Legese MH 

PROVIDER: S-EPMC11470727 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature