Juvenile hormone signaling is indispensable for late embryogenesis in ametabolous and hemimetabolous insects.

ABSTRACT:

Background

Juvenile hormone (JH) is an insect-exclusive hormone involved in regulating diverse aspects of insect physiology, and the evolution of its diverse function is widely interesting. Studying embryogenesis in basal wingless insects is important to understand the functional evolution of JH; however, experimental studies in this regard are scarce. In this study, we conducted CRISPR/Cas9-mediated knockout (KO) of genes involved in JH biosynthesis and signaling cascades in the ametabolous firebrat, Thermobia domestica. Additionally, we investigated whether the primitive action of JH is conserved in the hemimetabolous cricket, Gryllus bimaculatus.

Results

We observed that KO of JHAMT, CYP15A1, Met, and Kr-h1 resulted in embryonic lethality in T. domestica. Deprivation of J

SUBMITTER: Lv YN 

PROVIDER: S-EPMC11470741 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature