Single-cell transcriptome atlas revealed bronchoalveolar immune features related to disease severity in pediatric Mycoplasma pneumoniae pneumonia.

ABSTRACT: The mechanisms underlying protective immunity in mild Mycoplasma pneumoniae pneumonia (MPP) and the pathogenesis of severe MPP, characterized by dysregulated immune responses, remain unclear. Here, we performed single-cell RNA sequencing (scRNA-seq) to profile bronchoalveolar lavage fluid (BALF) samples from 13 healthy donors and 24 hospitalized pediatric patients with MPP, covering both mild and severe cases. Severe MPP patients exhibited high levels of exhausted T cells and M1-like macrophages, with the exhaustion of T cells attributed to persistent type I interferon signaling and inadequate assistance from CD4⁺ T cells. Significant cell-cell interactions between exhausted T cells and programmed death-ligand 1⁺ (PD-L1⁺) macrophages were detected in

SUBMITTER: Shen X 

PROVIDER: S-EPMC11471001 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature