Differential transcriptomic host responses in the early phase of viral and bacterial infections in human lung tissue explants ex vivo.

ABSTRACT:

Background

The first 24 h of infection represent a critical time window in interactions between pathogens and host tissue. However, it is not possible to study such early events in human lung during natural infection due to lack of clinical access to tissue this early in infection. We, therefore, applied RNA sequencing to ex vivo cultured human lung tissue explants (HLTE) from patients with emphysema to study global changes in small noncoding RNA, mRNA, and long noncoding RNA (lncRNA, lincRNA) populations during the first 24 h of infection with influenza A virus (IAV), Mycobacterium bovis Bacille Calmette-Guerin (BCG), and Pseudomonas aeruginosa.

Results

Pseudomonas aeruginosa caused the strongest expression changes and was the only pathogen that notably affected expression

SUBMITTER: Sohail A 

PROVIDER: S-EPMC11471021 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature