Novel cell spheroid culture method using Medaka dried fish powder.

ABSTRACT: Highly biocompatible microcarriers are culture materials designed to enhance the efficiency of cell spheroid culture. Typically, collagen or specially processed plastic materials serve as these microcarriers. In the context of cultured-cell-based food production, however, both collagen and plastic materials present challenges regarding their cost-effectiveness and edibility. A notable issue with collagen, especially when derived from fish scales, is its low denaturation temperature, making it unsuitable for use with mammalian cells unless cross-linked. To address this issue, our research pivoted towards utilizing dried fish, a rich source of proteins including collagen. For this study, Medaka fish were selected. The fish were dried, ground into fine particles, and then impregnated with eth

SUBMITTER: Sakumoto T 

PROVIDER: S-EPMC11471157 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature