Exploring immune response toward transplanted human kidney tissues assembled from organoid building blocks.

ABSTRACT: The increasing scarcity of organs and the significant morbidity linked to dialysis require the development of engineered kidney tissues from human-induced pluripotent stem cells. Integrative approaches that synergize scalable kidney organoid differentiation, tissue biomanufacturing, and comprehensive assessment of their immune response and host integration are essential to accomplish this. Here, we create engineered human kidney tissues composed of organoid building blocks (OBBs) and transplant them into mice reconstituted with allogeneic human immune cells. Tissue-infiltrating human immune cells are composed of effector T cells and innate cells. This immune infiltration leads to kidney tissue injury characterized by reduced microvasculature, enhanced kidney cell apoptosis, and an inflamma

SUBMITTER: Borges TJ 

PROVIDER: S-EPMC11471229 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature