Expression of stress responsive genes enables Limosilactobacillus reuteri to cross-protection against acid, bile salt, and freeze-drying.

ABSTRACT:

Introduction

Limosilactobacillus reuteri effectively colonizing the gut, secretes antimicrobial compounds and strengthens immune system function. Considering these health benefits, increasing its stress assessments efficiency could improve its commercial viability.

Methods

In this work, the resistance of L. reuteri FP41 to acid, bile salts, and freeze-drying was examined.

Results

The findings showed that strain FP41 demonstrated a strong resistance to acid/bile salt stresses. The transcriptome revealed a significant up-regulation of various stress response genes, including those related to membrane integrity, glutamine metabolism, OsmC family protein, ABC transporters, and chaperonin. Subsequent research demonstrated that overexpression of three stress r

SUBMITTER: Liu Z 

PROVIDER: S-EPMC11471561 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature