Robotic microinjection enables large-scale transgenic studies of Caenorhabditis elegans.

ABSTRACT: The nematode Caenorhabditis elegans is widely employed as a model organism to study basic biological mechanisms. However, transgenic C. elegans are generated by manual injection, which remains low-throughput and labor-intensive, limiting the scope of approaches benefitting from large-scale transgenesis. Here, we report a robotic microinjection system, integrating a microfluidic device capable of reliable worm immobilization, transfer, and rotation, for high-speed injection of C. elegans. The robotic system provides an injection speed 2-3 times faster than that of experts with 7-22 years of experience while maintaining comparable injection quality and only limited trials needed by users to become proficient. We further employ our system in a large-scale reverse genetic screen using multiple

SUBMITTER: Pan P 

PROVIDER: S-EPMC11471809 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature