Evolutionary engineering of methylotrophic E. coli enables fast growth on methanol.

ABSTRACT: As methanol can be derived from either CO₂ or methane, methanol economy can play an important role in combating climate change. In this scenario, rapid utilization of methanol by an industrial microorganism is the first and crucial step for efficient utilization of the C1 feedstock chemical. Here, we report the development of a methylotrophic E. coli strain with a doubling time of 3.5 hours under optimal conditions, comparable or faster than native model methylotrophs Methylorubrum extorquens AM1 (T_d~4hr) and Bacillus methanolicus at 37°C (T_d~5hr). To accomplish this, we develop a bacterial artificial chromosome (BAC) with dynamic copy number variation (CNV) to facilitate overcoming the formaldehyde-induced DNA-protein cross-linking (DPC) problem in the evo

SUBMITTER: Nieh LY 

PROVIDER: S-EPMC11471845 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature