Distinct Transcript-Level Expression Profiles and Unique Alternative Splicing in Inflammatory Myopathies.

ABSTRACT:

Objective

The pathogenesis of inflammatory myopathies is poorly understood and there is a need to dissect the transcriptome in more granular ways beyond gene expression.

Methods

We used a set of muscle RNA-sequencing data from different myositis subtypes grouped by their specific autoantibodies (n = 152). We quantified annotated RNA transcripts for each myositis subtype and identified uniquely expressed RNA as well as transcriptional similarities among myositis types. In addition, we quantified event-based alternative splicing with predicted protein changes. And finally, we searched for cryptic exons.

Results

We saw considerable overlap in RNA expression among subtypes. In addition, MADCAM1 was previously shown to be uniquely expressed in Mi-2 myositis; we discovered

SUBMITTER: Najjar R 

PROVIDER: S-EPMC11471943 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature