Snorkel-tag based affinity chromatography for recombinant extracellular vesicle purification.

ABSTRACT: Extracellular vesicles (EVs) are lipid nanoparticles and play an important role in cell-cell communications, making them potential therapeutic agents and allowing to engineer for targeted drug delivery. The expanding applications of EVs in next generation medicine is still limited by existing tools for scaling standardized EV production, single EV tracing and analytics, and thus provide only a snapshot of tissue-specific EV cargo information. Here, we present the Snorkel-tag, for which we have genetically fused the EV surface marker protein CD81, to a series of tags with an additional transmembrane domain to be displayed on the EV surface, resembling a snorkel. This system enables the affinity purification of EVs from complex matrices in a non-destructive form while maintaining EV characte

SUBMITTER: Bobbili MR 

PROVIDER: S-EPMC11472238 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature