LCMS-Metabolomic Profiling and Genome Mining of <i>Delftia lacustris</i> DSM 21246 Revealed Lipophilic Delftibactin Metallophores.

ABSTRACT: Bacteria have evolved various strategies to combat heavy metal stress, including the secretion of small molecules, known as metallophores. These molecules hold a potential role in the mitigation of toxic metal contamination from the environment (bioremediation). Herein, we employed combined comparative metabolomic and genomic analyses to study the metallophores excreted by Delftia lacustris DSM 21246. LCMS-metabolomic analysis of this bacterium cultured under iron limitation led to a suite of lipophilic metallophores exclusively secreted in response to iron starvation. Additionally, we conducted genome sequencing of the DSM 21246 strain using nanopore sequencing technology and employed antiSMASH to mine the genome, leading to the identification of a biosynthetic gene cluster (BGC) m

SUBMITTER: Ahmed MMA 

PROVIDER: S-EPMC11472818 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature