Sensitive and Enzyme-Free <i>Pseudomonas aeruginosa</i> Detection and Isolation via DNAzyme Cascade Triggered DNA Tweezer.

ABSTRACT: Effective isolation and sensitive detection of Pseudomonas aeruginosa (P. aeruginosa) is crucial for the early diagnosis and prognosis of various diseases, such as urinary tract infections. However, efficient isolation and simultaneous detection of P. aeruginosa remains a huge challenge. Herein, we depict a novel fluorescence assay for sensitive, enzyme-free detection of P. aeruginosa by integrating DNAzyme cascade-induced DNA tweezers and magnetic nanoparticles (MNPs)-based separation. The capture probe@MNPs is capable of accurately identifying target bacteria and transporting the bacteria signal to nucleic acid signals. Based on the DNAzyme cascade-induced DNA tweezers, the nucleic acid signals are extensively amplified, endowing the method with a high sensiti

SUBMITTER: Liu F 

PROVIDER: S-EPMC11473567 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature