Bioreceptors' immobilization by hydrogen bonding interactions and differential pulse voltammetry for completely label-free electrochemical biosensors.

ABSTRACT: Label-free electrochemical biosensors show great potential for the development of point-of-care devices (POCDs) for environmental and clinical applications. These sensors operate with shorter analysis times and are more economic than the labelled ones. Here, four completely label-free biosensors without electron transfer mediators were developed for hepatitis B virus (HBV) detection. The approach consisted in (i) the modification of gold surfaces with cysteamine (CT) or cysteine (CS) linkers, (ii) the subsequent antibody (Ab) immobilization, either directly by hydrogen bonding (HB) interactions or by covalent bonds (CB) using additional reagents, and (iii) measuring the biosensor response by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The electrod

SUBMITTER: Lopez-Marzo A 

PROVIDER: S-EPMC11473665 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature