A human ex vivo skin model breaking boundaries.

ABSTRACT: Ex vivo human skin models are valuable tools in skin research due to their physiological relevance. Traditionally, standard cultivation is performed in a cell culture incubator with a defined temperature of 37 °C and a specific atmosphere enriched with CO₂ to ensure media stability. Maintaining the model under these specific conditions limits its flexibility in assessing exposures to which the skin is exposed to in daily life, for example changes in atmospheric compositions. In this study we demonstrated that the foreskin-derived skin model can be successfully cultured at room temperature outside a CO₂ incubator using a CO₂-independent, serum-free media. Over a cultivation period of three days, the integrity of the tissue and the preservation of immune cell

SUBMITTER: Wurbs A 

PROVIDER: S-EPMC11473684 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature