Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts.

ABSTRACT: Bioluminescence imaging has become a valuable tool in biological research, offering several advantages over fluorescence-based techniques, including the absence of phototoxicity and photobleaching, along with a higher signal-to-noise ratio. Common bioluminescence imaging methods often require the addition of an external chemical substrate (luciferin), which can result in a decrease in luminescence intensity over time and limit prolonged observations. Since the bacterial bioluminescence system is genetically encoded for luciferase-luciferin production, it enables autonomous bioluminescence (auto-bioluminescence) imaging. However, its application to multiple reporters is restricted due to a limited range of color variants. Here, we report five-color auto-bioluminescence system named Nano-lan

SUBMITTER: Kusuma SH 

PROVIDER: S-EPMC11474039 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature