Chemical mapping of the surface interactome of PIEZO1 identifies CADM1 as a modulator of channel inactivation.

ABSTRACT: The propeller-shaped blades of the PIEZO1 and PIEZO2 ion channels partition into the plasma membrane and respond to indentation or stretching of the lipid bilayer, thus converting mechanical forces into signals that can be interpreted by cells, in the form of calcium flux and changes in membrane potential. While PIEZO channels participate in diverse physiological processes, from sensing the shear stress of blood flow in the vasculature to detecting touch through mechanoreceptors in the skin, the molecular details that enable these mechanosensors to tune their responses over a vast dynamic range of forces remain largely uncharacterized. To survey the molecular landscape surrounding PIEZO channels at the cell surface, we employed a mass spectrometry-based proteomic approach to capture and id

SUBMITTER: Koster AK 

PROVIDER: S-EPMC11474052 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature