MEnrich-seq: methylation-guided enrichment sequencing of bacterial taxa of interest from microbiome.

ABSTRACT: Metagenomics has enabled the comprehensive study of microbiomes. However, many applications would benefit from a method that sequences specific bacterial taxa of interest, but not most background taxa. We developed mEnrich-seq (in which 'm' stands for methylation and seq for sequencing) for enriching taxa of interest from metagenomic DNA before sequencing. The core idea is to exploit the self versus nonself differentiation by natural bacterial DNA methylation and rationally choose methylation-sensitive restriction enzymes, individually or in combination, to deplete host and background taxa while enriching targeted taxa. This idea is integrated with library preparation procedures and applied in several applications to enrich (up to 117-fold) pathogenic or beneficial bacteria from human urin

SUBMITTER: Cao L 

PROVIDER: S-EPMC11474163 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature