Proteome Dynamics in iPSC-Derived Human Dopaminergic Neurons.

ABSTRACT: Dopaminergic neurons participate in fundamental physiological processes and are the cell type primarily affected in Parkinson's disease. Their analysis is challenging due to the intricate nature of their function, involvement in diverse neurological processes, and heterogeneity and localization in deep brain regions. Consequently, most of the research on the protein dynamics of dopaminergic neurons has been performed in animal cells ex vivo. Here we use iPSC-derived human mid-brain-specific dopaminergic neurons to study general features of their proteome biology and provide datasets for protein turnover and dynamics, including a human axonal translatome. We cover the proteome to a depth of 9409 proteins and use dynamic SILAC to measure the half-life of more than 4300 proteins. We report un

SUBMITTER: Cavarischia-Rega C 

PROVIDER: S-EPMC11474371 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature