Direct Measurements of FLASH-Induced Changes in Intracellular Oxygenation.

ABSTRACT:

Purpose

The goal of our study was to characterize the dynamics of intracellular oxygen during application of radiation at conventional (CONV) and FLASH dose rates and obtain evidence for or against the oxygen depletion hypothesis as a mechanism of the FLASH effect.

Methods and materials

The measurements were performed by the phosphorescence quenching method using probe Oxyphor PtG4, which was delivered into the cellular cytosol by electroporation.

Results

Intracellular radiochemical oxygen depletion (ROD) g-value for a dose rate of 100 Gy/s in the normoxic range was found to be 0.58 ± 0.03 μM/Gy. Intracellular ROD g-values for FLASH and CONV dose rates in the normoxic range were found to be nearly equal. As in solution-based studies, intracellular ROD was found to exh

SUBMITTER: El Khatib M 

PROVIDER: S-EPMC11474819 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature