Diversity of microglial transcriptional responses during opioid exposure and neuropathic pain.

ABSTRACT:

Abstract

Microglia take on an altered morphology during chronic opioid treatment. This morphological change is broadly used to identify the activated microglial state associated with opioid side effects, including tolerance and opioid-induced hyperalgesia (OIH). Microglia display similar morphological responses in the spinal cord after peripheral nerve injury (PNI). Consistent with this observation, functional studies have suggested that microglia activated by opioids or PNI engage common molecular mechanisms to induce hypersensitivity. In this article, we conducted deep RNA sequencing (RNA-seq) and morphological analysis of spinal cord microglia in male mice to comprehensively interrogate transcriptional states and mechanistic commonality between multiple models of OIH and PNI. Af

SUBMITTER: Sypek EI 

PROVIDER: S-EPMC11474913 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature