Activation Pathways of Murine Macrophages by Lipophosphoglycan from Strains of <i>Leishmania major</i> (FV1 and LV39).

ABSTRACT: Lipophosphoglycan (LPG) is an important Leishmania virulence factor. It is the most abundant surface glycoconjugate in promastigotes, playing an important role in the interaction with phagocytic cells. While LPG is known to modulate the macrophage immune response during infection, the activation mechanisms triggered by this glycoconjugate have not been fully elucidated. This work investigated the role that LPGs purified from two strains of Leishmania major (FV1 and LV39) play in macrophage activation, considering the differences in their biochemical structures. Bone marrow-derived macrophages from BALB/c mice were stimulated with 10 μg/mL purified LPG from the LV39 and FV1 strains. We then measured the production of nitric oxide (NO) and cytokines, the expression of inducible

SUBMITTER: Mancur Santos V 

PROVIDER: S-EPMC11474971 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature