Error propagation in constraint-based modeling of Chinese hamster ovary cells.

ABSTRACT: Chinese hamster ovary (CHO) cells are the most popular mammalian cell factories for the production of glycosylated biopharmaceuticals. To further increase titer and productivity and ensure product quality, rational system-level engineering strategies based on constraint-based metabolic modeling, such as flux balance analysis (FBA), have gained strong interest. However, the quality of FBA predictions depends on the accuracy of the experimental input data, especially on the exchange rates of extracellular metabolites. Yet, it is not standard practice to devote sufficient attention to the accurate determination of these rates. In this work, we investigated to what degree the sampling frequency during a batch culture and the measurement errors of metabolite concentrations influence the accurac

SUBMITTER: Szeliova D 

PROVIDER: S-EPMC11475314 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature