Mismatch detection in homologous strand exchange amplified by hydrophobic effects.

ABSTRACT: In contrast to DNA replication and transcription where nucleotides are added and matched one by one, homologous recombination by DNA strand exchange tests whole sequences for complementarity, which requires elimination of mismatched yet thermodynamically stable intermediates. To understand the remarkable sequence specificity of homologous recombination, we have studied strand exchange between a 20-mer duplex containing one single mismatch (placed at varied positions) with the matching single strand in presence of poly(ethylene glycol) representing a semi-hydrophobic environment. A FRET-based assay shows that rates and yields of strand exchange from mismatched to matched strands rapidly increase with semi-hydrophobic co-solute concentration, contrasting previously observed general strand ex

SUBMITTER: Norden B 

PROVIDER: S-EPMC11475334 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature