Laherradurin Inhibits Colorectal Cancer Cell Growth by Induction of Mitochondrial Dysfunction and Autophagy Induction.

ABSTRACT: LAH, an acetogenin from the Annonaceae family, has demonstrated antitumor activity in several cancer cell lines and in vivo models, where it reduced the tumor size and induced programmed cell death. We focused on the effects of LAH on mitochondrial dynamics, mTOR signaling, autophagy, and apoptosis in colorectal cancer (CRC) cells to explore its anticancer potential.

Methods

CRC cells were treated with LAH, and its effects on mitochondrial respiration and glycolysis were measured using Seahorse XF technology. The changes in mitochondrial dynamics were observed through fluorescent imaging, while Western blot analysis was used to examine key autophagy and apoptosis markers.

Results

LAH significantly inhibited mitochondrial complex I activity, inducing ATP depletion and a compen

SUBMITTER: Delgado-Waldo I 

PROVIDER: S-EPMC11475353 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature