Intracellular osteopontin potentiates the immunosuppressive activity of mesenchymal stromal cells.

ABSTRACT:

Introduction

Mesenchymal stromal cell (MSC)-based cell therapy is a promising approach for various inflammatory disorders based on their immunosuppressive capacity. Osteopontin (OPN) regulates several cellular functions including tissue repair, bone metabolism and immune reaction. However, the biological function of OPN in regulating the immunosuppressive capacity of MSCs remains elusive.

Objectives

This study aims to highlight the underlying mechanism of the proinflammatory cytokines affect the therapeutic ability of MSCs through OPN.

Methods

MSCs in response to the proinflammatory cytokines were collected to determine the expression profile of OPN. In vitro T-cell proliferation assays and gene editing were performed to check the role and mechanisms of OPN in regulat

SUBMITTER: Yang W 

PROVIDER: S-EPMC11475537 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature