CD81-guided heterologous EVs present heterogeneous interactions with breast cancer cells.

ABSTRACT:

Background

Extracellular vesicles (EVs) are cell-secreted particles conceived as natural vehicles for intercellular communication. The capacity to entrap heterogeneous molecular cargoes and target specific cell populations through EV functionalization promises advancements in biomedical applications. However, the efficiency of the obtained EVs, the contribution of cell-exposed receptors to EV interactions, and the predictability of functional cargo release with potential sharing of high molecular weight recombinant mRNAs are crucial for advancing heterologous EVs in targeted therapy applications.

Methods

In this work, we selected the popular EV marker CD81 as a transmembrane guide for fusion proteins with a C-terminal GFP reporter encompassing or not Trastuzumab light chains

SUBMITTER: Gurrieri E 

PROVIDER: S-EPMC11475557 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature