Endothelial Myosin IIA Is Required for the Maintenance of Blood-Brain Barrier Integrity.

ABSTRACT: Brain endothelial cells (ECs) are essential elements of the blood-brain barrier (BBB), maintaining its integrity through both paracellular junctions and transcellular transport systems. Myosin IIA, a multifunctional protein, plays a significant role in various cellular processes, including cytoskeletal maintenance, cell division, and signal transduction. While Myosin IIA has been implicated in bleeding and ischemic stroke, its role in regulating BBB integrity under physiological conditions remains unclear. In this study, we investigated the impact of Myosin IIA deficiency on BBB integrity using intravenous tracer injections and models of epilepsy. Flow cytometry, Western blot, and real-time PCR were employed to isolate brain cells and assess changes in protein and mRNA levels. Additionally

SUBMITTER: Deng Y 

PROVIDER: S-EPMC11475711 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature