Combined PET Radiotracer Approach Reveals Insights into Stromal Cell-Induced Metabolic Changes in Pancreatic Cancer In Vitro and In Vivo.

ABSTRACT: Background/Objective Pancreatic stellate cells (PSCs) in pancreatic adenocarcinoma (PDAC) are producing extracellular matrix, which promotes the formation of a dense fibrotic microenvironment. This makes PDAC a highly heterogeneous tumor-stroma-driven entity, associated with reduced perfusion, limited oxygen supply, high interstitial fluid pressure, and limited bioavailability of therapeutic agents. Methods In this study, spheroid and tumor xenograft models of human PSCs and PanC-1 cells were characterized radiopharmacologically using a combined positron emission tomography (PET) radiotracer approach. [¹⁸F]FDG, [¹⁸F]FMISO, and [¹⁸F]FAPI-74 were employed to monitor metabolic activity, hypoxic metabolic state, and functional expression of fibrob

SUBMITTER: Doctor A 

PROVIDER: S-EPMC11475921 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature