Evolutionary origins and innovations sculpting the mammalian PRPS enzyme complex.

ABSTRACT: The phosphoribosyl pyrophosphate synthetase (PRPS) enzyme conducts a chokepoint reaction connecting central carbon metabolism and nucleotide production pathways, making it essential for life^1,2. Here, we show that the presence of multiple PRPS-encoding genes is a hallmark trait of eukaryotes, and we trace the evolutionary origins and define the individual functions of each of the five mammalian PRPS homologs - three isozymes (one testis-restricted)^3,4 and two non-enzymatic associated proteins (APs)^5,6 - which we demonstrate operate together as a large molecular weight complex capable of attaining a heterogeneous array of functional multimeric configurations. Employing a repertoire of isogenic fibroblast clones in all viable individual or combinatorial assem

SUBMITTER: Karki BR 

PROVIDER: S-EPMC11476008 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature