Dysregulation of pseudouridylation in small RNAs contributes to papillary thyroid carcinoma metastasis.

ABSTRACT:

Background

Previous studies have indicated that ψ-modified small RNAs play crucial roles in tumor metastasis. However, the ψ-modified small RNAs during metastasis of PTC are still unclear.

Methods

We compared the pseudouridine synthase 7 (PUS7) alteration between metastatic and non-metastatic PTCs, and investigated its correlation with clinicopathological features. Additionally, we employed a small RNA ψ modification microarray to examine the small RNA ψ modification profile in both metastatic and non-metastatic PTCs, as well as paired paracancerous tissues. The key molecule involved in ψ modification, pre-miR-8082, was identified and found to regulate the expression of CD47. Experiments in vitro were conducted to further investigate the function of PUS7 and CD47 in PTC.

SUBMITTER: Wang X 

PROVIDER: S-EPMC11476189 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature