Metabolic Engineering and Process Intensification for Muconic Acid Production Using <i>Saccharomyces cerevisiae</i>.

ABSTRACT: The efficient production of biobased organic acids is crucial to move to a more sustainable and eco-friendly economy, where muconic acid is gaining interest as a versatile platform chemical to produce industrial building blocks, including adipic acid and terephthalic acid. In this study, a Saccharomyces cerevisiae platform strain able to convert glucose and xylose into cis,cis-muconic acid was further engineered to eliminate C2 dependency, improve muconic acid tolerance, enhance production and growth performance, and substantially reduce the side production of the intermediate protocatechuic acid. This was achieved by reintroducing the PDC5 gene and overexpression of QDR3 genes. The improved strain was integrated in low-pH fed-batch fermentations at biore

SUBMITTER: Tonjes S 

PROVIDER: S-EPMC11476194 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature