Unveiling Insights into the Whole Genome Sequencing of <i>Mycobacterium</i> spp. Isolated from Siamese Fighting Fish (<i>Betta splendens</i>).

ABSTRACT: This study aims to genomically elucidate six isolates of rapidly growing non-tuberculous mycobacteria (RGM) derived from Siamese fighting fish (Betta splendens). These isolates had previously undergone phenotypic and biochemical characterization, antibiotic susceptibility testing, and in vivo virulence assessment. Initial DNA barcoding using the 16S rRNA sequence assigned these six isolates to five different species, namely Mycobacterium chelonae (BN1983), M. cosmeticum (BN1984 and N041), M. farcinogenes (SNSK5), M. mucogenicum (BN1956), and M. senegalense (BN1985). However, the identification relied solely on the highest percent identity of the 16S rRNA gene, raising concerns about the taxonomic ambiguity of these species. Comprehensive whole geno

SUBMITTER: Dinh-Hung N 

PROVIDER: S-EPMC11476334 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature