Full-Length Transcriptome Profile of Apis cerana Revealed by Nanopore Sequencing.

ABSTRACT: The Asian honey bee (Apis cerana) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three pooled RNA samples from the queen, drone, and worker bees of A. cerana and performed full-length RNA sequencing using Nanopore single-molecule sequencing technology. Ultimately, we obtained 160,811 full-length transcript sequences from 19,859 genes, with 141,189 being novel transcripts, of which 130,367 were functionally annotated. We detected 520, 324, and 1823 specifically expressed transcripts in the queen, worker, and drone bees, respectively. Furthermore, we identifi

SUBMITTER: Hu XF 

PROVIDER: S-EPMC11476444 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature