Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients.

ABSTRACT:

Background

Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively.

Results

In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × 10⁵ cells stimulation was superior to 2 × 10⁶ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditio

SUBMITTER: Wang L 

PROVIDER: S-EPMC11476462 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature