PH-Responsive Collagen Hydrogels Prepared by UV Irradiation in the Presence of Riboflavin.

ABSTRACT: This study reveals the pH-responsive behavior of collagen hydrogels prepared using ultraviolet (UV) irradiation with riboflavin as a photosensitizer. By varying the UV exposure time, we modulated the crosslinking density, thereby influencing the mechanical properties and pH responsiveness. Rheological analysis confirmed successful network formation, whereas swelling studies revealed significant pH-dependent behavior, with maximum swelling at a pH of four and minimal swelling above a pH of six, demonstrating partial reversibility over multiple pH cycles. Mechanical testing showed a pH-dependent elastic modulus, which increased 10 fold from a pH of 6 to 10. Fibroblast proliferation assays confirmed the biocompatibility of the hydrogels, with cell growth positively correlating with the UV exp

SUBMITTER: Setoyama S 

PROVIDER: S-EPMC11476811 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature