Use of Bacterial Toxin-Antitoxin Systems as Biotechnological Tools in Plants.

ABSTRACT: Toxin-antitoxin (TA) systems in bacteria are key regulators of the cell cycle and can activate a death response under stress conditions. Like other bacterial elements, TA modules have been widely exploited for biotechnological purposes in diverse applications, such as molecular cloning and anti-cancer therapies. However, their use in plants has been limited, leaving room for the development of new approaches. In this study, we examined two TA systems previously tested in plants, MazEF and YefM-YoeB, and identified interesting differences between them, likely related to their modes of action. We engineered modifications to these specific modules to transform them into molecular switches that can be activated by a protease, inducing necrosis in the plant cells where they are expressed. Final

SUBMITTER: Rodamilans B 

PROVIDER: S-EPMC11476816 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature