Signature Proteins in Small Extracellular Vesicles of Granulocytes and CD4⁺ T-Cell Subpopulations Identified by Comparative Proteomic Analysis.

ABSTRACT: Several studies have described the proteomic profile of different immune cell types, but only a few have also analysed the content of their delivered small extracellular vesicles (sEVs). The aim of the present study was to compare the protein signature of sEVs delivered from granulocytes (i.e., neutrophils and eosinophils) and CD4⁺ T cells (i.e., TH1, TH2, and TH17) to identify potential biomarkers of the inflammatory profile in chronic inflammatory diseases. Qualitative (DDA) and quantitative (DIA-SWATH) analyses of in vitro-produced sEVs revealed proteome variations depending on the cell source. The main differences were found between granulocyte- and TH cell-derived sEVs, with a higher abundance of antimicrobial proteins (e.g., LCN2, LTF, MPO) in granulocyte-derived sEVs and

SUBMITTER: Vazquez-Mera S 

PROVIDER: S-EPMC11476868 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature