Structural Insights and Catalytic Mechanism of 3-Hydroxybutyryl-CoA Dehydrogenase from <i>Faecalibacterium Prausnitzii</i> A2-165.

ABSTRACT: Atopic dermatitis (AD) is characterized by a T-helper cell type 2 (Th2) inflammatory response leading to skin damage with erythema and edema. Comparative fecal sample analysis has uncovered a strong correlation between AD and Faecalibacterium prausnitzii strain A2-165, specifically associated with butyrate production. Therefore, understanding the functional mechanisms of crucial enzymes in the butyrate pathway, such as 3-hydroxybutyryl-CoA dehydrogenase of A2-165 (A2HBD), is imperative. Here, we have successfully elucidated the three-dimensional structure of A2HBD in complex with acetoacetyl-CoA and NAD⁺ at a resolution of 2.2Å using the PAL-11C beamline (third generation). Additionally, X-ray data of A2HBD in complex with acetoacetyl-CoA at a resolution of 1.9 Å were col

SUBMITTER: Yang J 

PROVIDER: S-EPMC11476959 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature